Insertion junction: LMJ.RY0402.103001_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.103001
Chromosome:chromosome 6
Location:6351099
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g292000 (1 of 1) PF00790//PF03127 - VHS domain (VHS) // GAT domain (GAT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTACGCCTGTGTCCTCCAACCTCCCACA
Internal bar code:CTAAGCGATGTGATAGGACGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1895
LEAP-Seq percent confirming:98.8589
LEAP-Seq n confirming:1646
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:AATCTTGCTCCCACAAGGTG
Suggested primer 2:AGGGAGTGTGAATGGGAGTG