| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103108 |
| Chromosome: | chromosome 3 |
| Location: | 89647 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143887 | TSR1 | (1 of 1) 6.1.1.19 - Arginine--tRNA ligase / Arginyl-tRNA synthetase; Arginyl-tRNA Synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGATCGGTTCGTACAGGCACCCCCGCCT |
| Internal bar code: | ATCGTCATCGGTTGCTACTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 99.0783 |
| LEAP-Seq n confirming: | 215 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGTGGATGGAGCGTTTT |
| Suggested primer 2: | TGGTCCGGGAAGATGAGTAG |