Insertion junction: LMJ.RY0402.103679_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.103679
Chromosome:chromosome_10
Location:4437166
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre10.g451600 antisense intron|outside_mRNA

Insertion site details

Flanking sequence (orientation from cassette outwards):TGAAACTCGCCGCCCGCTTGATCGTTTCCA
Internal bar code:TAATCTTCCGTTCGATACGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1073
LEAP-Seq percent confirming:99.6899
LEAP-Seq n confirming:6429
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAACATCTGCCTCACAAGCA
Suggested primer 2:CGAGAATGTCACTCAGGCAA