| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103682 |
| Chromosome: | chromosome 11 |
| Location: | 3152434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479300 | SRH20 | SNF2-related DNA/RNA helicase; (1 of 1) K15083 - DNA repair protein RAD16 (RAD16) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGATTCGGATGAGGATGAGGAGGACGA |
| Internal bar code: | GCAGCTGGAGCCTCCGTTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 142 |
| LEAP-Seq percent confirming: | 98.1982 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACACCCCATCAAC |
| Suggested primer 2: | ACACACACACACACACACCG |