| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103735 |
| Chromosome: | chromosome_16 |
| Location: | 1162263 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g650450 | FAP219 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTCCCTGGATTGAGGGCCACAGTCTCGT |
| Internal bar code: | AGGGAGACGTGTGTTTGTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.6884 |
| LEAP-Seq n confirming: | 9917 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGCTGAAGAGTTTTGGG |
| Suggested primer 2: | ACCTTCATGAATCCAGCCAC |