| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.104596 |
| Chromosome: | chromosome 9 |
| Location: | 5834007 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402219 | LCI3 | Low-CO2-inducible protein | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGCAGAATTCGAGTCCGTACGGATGTA |
| Internal bar code: | ATGGCTCTTGGGCTAATATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 98.8095 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAGCACGTAGGCACCTT |
| Suggested primer 2: | CAGGGATGGATCAGGAGAAA |