| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104962 |
| Chromosome: | chromosome 5 |
| Location: | 428838 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232751 | (1 of 2) PTHR23518:SF2 - MULTIDRUG RESISTANCE PROTEIN MDTH | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGATGTACGAGTGCCTTGGCATGGCAG |
| Internal bar code: | TCCCACCGCTATCGCAGCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 98.1765 |
| LEAP-Seq n confirming: | 4738 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTACTGCCACTGCTACTG |
| Suggested primer 2: | TCATCACCATCCTCTCTCCC |