| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105093 |
| Chromosome: | chromosome 12 |
| Location: | 3814277 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515350 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGTCCGCACCCAGCACTTCACACTGC |
| Internal bar code: | TTATGTGGGCACCTCCCTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 917 |
| LEAP-Seq percent confirming: | 94.0439 |
| LEAP-Seq n confirming: | 600 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCGTGTTTGCATGTTTT |
| Suggested primer 2: | GAAACACAAGGAAATGCGGT |