| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105329 |
| Chromosome: | chromosome 2 |
| Location: | 2532381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092500 | UPF1 | (1 of 1) PTHR10887//PTHR10887:SF378 - DNA2/NAM7 HELICASE FAMILY // REGULATOR OF NONSENSE TRANSCRIPTS 1 HOMOLOG; UPF1-like RNA helicase, NMD protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGCAGGTGCGGGCTGCGGCTGCGGGGG |
| Internal bar code: | CTCCCGAAACTATCACCAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 746 |
| LEAP-Seq percent confirming: | 96.0042 |
| LEAP-Seq n confirming: | 12902 |
| LEAP-Seq n nonconfirming: | 537 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGAGATGTGGAAGACCA |
| Suggested primer 2: | GTGGAGAAGGAATGACGAGC |