| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105390 |
| Chromosome: | chromosome 11 |
| Location: | 1653973 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467772 | CYCD1 | D-type cyclin; (1 of 10) PTHR10177 - CYCLINE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCGTTAACGCTAACGCCGAGGCGCTAC |
| Internal bar code: | TTGTGCTTGGCGAACACGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 98.9681 |
| LEAP-Seq n confirming: | 3069 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGTCTGCGACGACGAAT |
| Suggested primer 2: | GCGTAGCAAGAAGGGACAAG |