| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105616 |
| Chromosome: | chromosome 13 |
| Location: | 2469686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGACCAACTGACGGAAAATAACGAGCGT |
| Internal bar code: | CGTCTGTAGCGGTCTGGTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1065 |
| LEAP-Seq percent confirming: | 99.1645 |
| LEAP-Seq n confirming: | 1899 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCTCTGCTGCATGTGTA |
| Suggested primer 2: | CCGTCTGCTGTCTTCTAGGG |