| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105856 |
| Chromosome: | chromosome 6 |
| Location: | 5003429 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280050 | ROC86,XRN1 | (1 of 1) K12618 - 5'-3' exoribonuclease 1 (XRN1, SEP1, KEM1); 5' to 3' exoribonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCGCTTGAGTACTGGCGCTAGCTTTTG |
| Internal bar code: | GGTATACTGATGGCGTTAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 98.6893 |
| LEAP-Seq n confirming: | 4894 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCGACTTCTACCCCGAC |
| Suggested primer 2: | AACTTGGTGAGGAATGTGGC |