Insertion junction: LMJ.RY0402.106342_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.106342
Chromosome:chromosome 11
Location:769209
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467634 (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGACAGCGAGCGGAGGTCCAACGTCTGGA
Internal bar code:GTCTGTCGGTGGTATCAAGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:529
LEAP-Seq percent confirming:95.4377
LEAP-Seq n confirming:1548
LEAP-Seq n nonconfirming:74
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TAAACTGGGACCTCCGTGAC
Suggested primer 2:GAACCCAACCCAAATGTACG