| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106349 |
| Chromosome: | chromosome 13 |
| Location: | 3271927 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586050 | (1 of 1) PTHR13833:SF49 - NHL REPEAT-CONTAINING PROTEIN 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCCATCCGCACCTGTCCTTCTCGTTGT |
| Internal bar code: | GTTTTTCCGACTGAACCGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 97.9363 |
| LEAP-Seq n confirming: | 2183 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTGACTGCTGTGTTTGT |
| Suggested primer 2: | TGGTTTTGGTTTAGCTTGGG |