Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.106619 |
Chromosome: | chromosome_14 |
Location: | 3724276 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g632050 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGAGGAGGAACAGAACTCGATGGTTTGGA |
Internal bar code: | ACGAATGGGCGTGGCCCGCATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 279 |
LEAP-Seq percent confirming: | 99.1049 |
LEAP-Seq n confirming: | 775 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTGCATCTGGCTGCTTAC |
Suggested primer 2: | TTCCTTGGATGGGTGATGAT |