| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106644 |
| Chromosome: | chromosome 1 |
| Location: | 7338665 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052750 | (1 of 1) PF06011//PF13418 - Transient receptor potential (TRP) ion channel (TRP) // Galactose oxidase, central domain (Kelch_4); Transient receptor potential ion channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCGTACATGAATCCGTACGACACGCCG |
| Internal bar code: | GAAAACGGGGTAGTCCGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 99.3939 |
| LEAP-Seq n confirming: | 1312 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGTGAGTTGTCGGTTTT |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |