| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.106646 |
| Chromosome: | chromosome 13 |
| Location: | 5018204 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606750 | (1 of 1) PTHR12447:SF0 - ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13C | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTCAGACATGATGCCGTACGACGGCAG |
| Internal bar code: | ATTCATGGATGAGAGCTGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 59.528 |
| LEAP-Seq n confirming: | 1362 |
| LEAP-Seq n nonconfirming: | 926 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCATGCTCTCACAGGAC |
| Suggested primer 2: | CTCCTGGTTCTTCAGCTTGG |