| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.106894 |
| Chromosome: | chromosome_2 |
| Location: | 6008754 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g112300 | CAL3,MOT15 | Calpain family cysteine protease | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTACTGCGTGTACTCGCGCGTGTCAAACA |
| Internal bar code: | GGATCTATGTCTCGATGCACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 75.4204 |
| LEAP-Seq n confirming: | 583 |
| LEAP-Seq n nonconfirming: | 190 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGGTGCTGTCTTGCCTT |
| Suggested primer 2: | GAACAGGTCATGCTCTGCAA |