Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.106894 |
Chromosome: | chromosome_9 |
Location: | 1193424 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g399911 | CDC20 | Activator and specificity subunit of anaphase promoting complex | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATCGCGCTTTCGTACGTCCCTGGACCTGC |
Internal bar code: | CGTCAGTCGTTTCTCAACTCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 581 |
LEAP-Seq percent confirming: | 40.9182 |
LEAP-Seq n confirming: | 820 |
LEAP-Seq n nonconfirming: | 1184 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTACCTGGAGGGTGAAAGG |
Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |