| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106903 |
| Chromosome: | chromosome 13 |
| Location: | 1655069 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573900 | NSS3 | (1 of 1) PTHR11819//PTHR11819:SF159 - SODIUM/SOLUTE SYMPORTER // SUBFAMILY NOT NAMED; Sodium:solute symporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGGAGCAAACCGTGCATTCGCGACTGCA |
| Internal bar code: | CTCGCCCAGACCCAGATGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 93.6864 |
| LEAP-Seq n confirming: | 460 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGGCCTAGGCTTCCTC |
| Suggested primer 2: | CCGTATGGTGACGTCTGATG |