Insertion junction: LMJ.RY0402.107467_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.107467
Chromosome:chromosome 12
Location:824876
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g495951 CYN8,CYN20C,CYN20-3,ROC4 (1 of 4) K03768 - peptidyl-prolyl cis-trans isomerase B (cyclophilin B) [EC:5.2.1.8] (PPIB, ppiB); Cyclophilin 20-3 3'UTR|outside_mRNA
Cre12.g495952 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAATCAACTACAATCAACTTGTTCCAGT
Internal bar code:ACCCGGCATGCATCCCCGGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:807
LEAP-Seq percent confirming:99.3578
LEAP-Seq n confirming:5570
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTTGCTCTCCGATCCAT
Suggested primer 2:AACTGCCCCAAACTATGCAC