Insertion junction: LMJ.RY0402.107526_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.107526
Chromosome:chromosome 9
Location:4131964
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393765 LMR1 (1 of 14) PF01476 - LysM domain (LysM); Predicted protein with LysM domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGGGATCGGCATACGCTAGCCCGGACTC
Internal bar code:TTCGCGGCCCACGTTTGCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:890
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:175
LEAP-Seq n nonconfirming:70
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGCCCAGAGCTCATATCT
Suggested primer 2:GAATATCTAGCGCGGACAGC