Insertion junction: LMJ.RY0402.107526_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.107526
Chromosome:chromosome 9
Location:4131972
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393765 LMR1 (1 of 14) PF01476 - LysM domain (LysM); Predicted protein with LysM domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACCAGAACCAGTCCACCGAGTGCGGAGG
Internal bar code:CGGGAGAATAAGGGGATTGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:490
LEAP-Seq percent confirming:98.8657
LEAP-Seq n confirming:3748
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGCCCAGAGCTCATATCT
Suggested primer 2:GAATATCTAGCGCGGACAGC