Insertion junction: LMJ.RY0402.108038_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.108038
Chromosome:chromosome 13
Location:1311709
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g571200 HKR5,HIK5,HIK (1 of 1) IPR000014//IPR001789//IPR003594//IPR003661//IPR004358//IPR005467//IPR011006 - PAS domain // Signal transduction response regulator, receiver domain // Histidine kinase-like ATPase, C-terminal domain // Signal transduction histidine kinase, dimerisation/phosphoacceptor domain // Signal transduction histidine kinase-related protein, C-terminal // Histidine kinase domain // CheY-like superfamily; Histidine kinase response regulator of two-component system intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTACACACGTGTCTTTGCGCAACGTTTTC
Internal bar code:ATTCTGTCATCGGTGTGACACG

Confirmation - LEAP-Seq

LEAP-Seq distance:598
LEAP-Seq percent confirming:98.9518
LEAP-Seq n confirming:944
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGTGGCTCTAGTGAGAAC
Suggested primer 2:CGTGTCTAGGGTGGTGAGGT