| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108183 |
| Chromosome: | chromosome 12 |
| Location: | 3052891 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500550 | (1 of 2) PTHR10579 - CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAATAAAGTCATGACTGCAACCTGCACCT |
| Internal bar code: | GTAGGCAATGTCGGTGGGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 96.9736 |
| LEAP-Seq n confirming: | 3717 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTCCGGCCAGTCTGTAT |
| Suggested primer 2: | CGCTGGTGGTGATGATGTAG |