Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.108407 |
Chromosome: | chromosome_12 |
Location: | 2012853 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g510200 | BLZ9 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCCATCCACATAAACCTTGAGTCGGGTCG |
Internal bar code: | CAAATGAGTAAGTCCAAGCTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 732 |
LEAP-Seq percent confirming: | 99.428 |
LEAP-Seq n confirming: | 2955 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTTCTTCTCATCACGACGC |
Suggested primer 2: | GTATCACCCGCCTATGCACT |