| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108500 |
| Chromosome: | chromosome 13 |
| Location: | 3147886 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585150 | EIF6A,EIF6 | (1 of 1) K03264 - translation initiation factor 6 (EIF6); Eukaryotic translation initiation factor 6 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTGGAATAAACAAGGCGGAGTATTGCA |
| Internal bar code: | CCAGCGGGGGCAGCTGAAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 99.6669 |
| LEAP-Seq n confirming: | 1197 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTCGCAGTTGCCTTAC |
| Suggested primer 2: | CAGTCGTTCGCTACTAGCCC |