| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108795 |
| Chromosome: | chromosome 12 |
| Location: | 8172746 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550900 | SSD3 | Sterol sensing domain protein; (1 of 1) K12385 - Niemann-Pick C1 protein (NPC1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCAAACTTCACGCACACCCCCCGAGCA |
| Internal bar code: | TATTCGGGATTCCCGGCTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 97.8923 |
| LEAP-Seq n confirming: | 418 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATACAAGCAGGGGAATCA |
| Suggested primer 2: | GGCGGCTAGACGAGTGTAAG |