| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108932 |
| Chromosome: | chromosome 13 |
| Location: | 129307 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562850 | THF1,PSB29 | Thylakoid Formation 1; (1 of 1) PTHR34793:SF1 - PROTEIN THYLAKOID FORMATION 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCTGGGTAGGGATGTGGGTGCGCGCAT |
| Internal bar code: | CATCCTAATTAGTCTCGAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 257 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTACAGACGGGGTAAGA |
| Suggested primer 2: | GAGGGGTTGAGTGCACGTAT |