| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108959 |
| Chromosome: | chromosome 17 |
| Location: | 392408 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698750 | RAA12,OPR104 | psaA mRNA trans-splicing factor of subcomplex I; (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCTGCCTTGCACCGCGCCTCGCTGTAC |
| Internal bar code: | CGGCCGACTGGTCGGGGAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 124 |
| LEAP-Seq percent confirming: | 98.6928 |
| LEAP-Seq n confirming: | 151 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACATATAGCGGCGGCAC |
| Suggested primer 2: | ATCAGGTAGAAGGAGGCGGT |