| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108987 |
| Chromosome: | chromosome_1 |
| Location: | 7070921 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g051050 | FAP225 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCCTGCATGAGACCGACGCTGCCAGTGGC |
| Internal bar code: | ATGTCGGTCAAGTTCTTGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 99.6844 |
| LEAP-Seq n confirming: | 12002 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATACTGCGCGATAACTA |
| Suggested primer 2: | AAGGACAGCACGGAGAAGAA |