| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109016 |
| Chromosome: | chromosome 9 |
| Location: | 3030232 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387400 | POLZ1 | DNA polymerase zeta; (1 of 1) K02350 - DNA polymerase zeta (POLZ1, rev3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAAGCCAACTTGGCACCGCAGTTGCAAG |
| Internal bar code: | CGGTAGGGCACTAATGTCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 124 |
| LEAP-Seq percent confirming: | 98.6509 |
| LEAP-Seq n confirming: | 1170 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCATTATCGTTCACCTG |
| Suggested primer 2: | TTCATTCTCCTGCTCCTGCT |