| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109029 |
| Chromosome: | chromosome_12 |
| Location: | 822632 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g495951 | CYN20-3,CYN8 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGATGTGCATGGTGAAGCCGAGTTTCGG |
| Internal bar code: | GACCTTCCGCGTGGGCTCACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 98.8191 |
| LEAP-Seq n confirming: | 6025 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTTCCTCCCCTCTTCAC |
| Suggested primer 2: | AACCAACAGCAATCAATCCC |