| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109949 |
| Chromosome: | chromosome 12 |
| Location: | 5095711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526701 | (1 of 7) PTHR10110 - SODIUM/HYDROGEN EXCHANGER | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAATAGCAACGCCCCCCCCCCCTTGCAC |
| Internal bar code: | GATGTCTGAGGAGCAGTTTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 98.164 |
| LEAP-Seq n confirming: | 1604 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACGTGATGCGAGAGTGC |
| Suggested primer 2: | CAAGGGCTACTCACCAGCTC |