| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110189 |
| Chromosome: | chromosome 17 |
| Location: | 2878041 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719500 | AOF9 | (1 of 2) 1.3.99.29 - Phytoene desaturase (zeta-carotene-forming) / 2-step phytoene desaturase; Flavin-containing amine oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGATGCAGGTGCGTGCGTGCGTGGCCA |
| Internal bar code: | GACTGGGGGGGACTGATGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 84.8098 |
| LEAP-Seq n confirming: | 3255 |
| LEAP-Seq n nonconfirming: | 583 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGCTGGGGATAATGCAC |
| Suggested primer 2: | CCTCCTTCCATCCTCCTTTC |