| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110700 |
| Chromosome: | chromosome 12 |
| Location: | 9168791 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542250 | TUB1,RSP19 | (1 of 2) K07375 - tubulin beta (TUBB); beta-1 tubulin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCACTCTCTGGGTGGTGGCACTGGCTC |
| Internal bar code: | ACTTCAATACTCTAACTTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 985 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACTTGACGTTGTTGGGG |
| Suggested primer 2: | AGCTTGGCTTCTTGTCCAAA |