| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110792 |
| Chromosome: | chromosome 4 |
| Location: | 187017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTACTTGGCCATCTGTCGGAATGAGGG |
| Internal bar code: | GGTTGGGTCTTCAGCAAAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 99.3784 |
| LEAP-Seq n confirming: | 1279 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAACAGCAGAATGGGGAC |
| Suggested primer 2: | CCAGTGCAATACTGAAGCGA |