| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110885 |
| Chromosome: | chromosome 17 |
| Location: | 3633810 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726350 | (1 of 1) IPR000104//IPR002794 - Antifreeze protein, type I // Protein of unknown function DUF92, TMEM19 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTATTTGTGTTTGAGGGCGATGTTCGT |
| Internal bar code: | GCAGAGGAGTCCAAGGGTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 96.7493 |
| LEAP-Seq n confirming: | 2381 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGGTTTTTGAGGGGAG |
| Suggested primer 2: | CACAGCTTCTGGGTGACTGA |