Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.111357 |
Chromosome: | chromosome 14 |
Location: | 499791 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g611300 | COP3,CHR1,CSOA | (1 of 7) PF01036 - Bacteriorhodopsin-like protein (Bac_rhodopsin); Channelrhodopsin 1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACCCTGCCGTCCATAGCAAGCAGTGCA |
Internal bar code: | CCGTCCGGGTCAGGCCGGGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 703 |
LEAP-Seq percent confirming: | 99.1624 |
LEAP-Seq n confirming: | 3078 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCACACCAGCTGCTCACAT |
Suggested primer 2: | TCCCCTGTCACTTTGACTCC |