| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112008 |
| Chromosome: | chromosome 6 |
| Location: | 1117268 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256850 | SND1B | SAND domain-encoding short ORF; (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGCAGTACCTGCGTGGGATTGCGGCCT |
| Internal bar code: | TTCTTACGGGACATTCATATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2924 |
| LEAP-Seq percent confirming: | 97.2746 |
| LEAP-Seq n confirming: | 464 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGACCACACTGACGAAGC |
| Suggested primer 2: | ATTCGTGTTTAGTGCGGACC |