| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112048 |
| Chromosome: | chromosome 13 |
| Location: | 2468455 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580000 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCCCCCGCGACGCAGCCGACTTACGGA |
| Internal bar code: | TCATTTCTCCTGTCCTATAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 98.1217 |
| LEAP-Seq n confirming: | 2403 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCTATCCCTTCCCTCAC |
| Suggested primer 2: | TACACATGCAGCAGAGGGAG |