Insertion junction: LMJ.RY0402.112165_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.112165
Chromosome:chromosome 12
Location:9463193
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g539650 (1 of 1) PF00530//PF08016 - Scavenger receptor cysteine-rich domain (SRCR) // Polycystin cation channel (PKD_channel) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCACCCCCTCCCTGCCCCTACCTCCCT
Internal bar code:CGGCTTGGTGGCGCGACGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:130
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTCCTTCAGTTTAAGCC
Suggested primer 2:ACATTGGGAACGGGATACAA