| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.113191 |
| Chromosome: | chromosome 6 |
| Location: | 4118275 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278221 | TSSP1,TPS1,TSPSP1 | (1 of 1) 2.4.1.15//3.1.3.12 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / UDP-glucose--glucose-phosphate glucosyltransferase // Trehalose-phosphatase / Trehalose 6-phosphate phosphatase; Trehalose-6-phosphate synthase/phosphatase, class I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGGTGTGAAGCGGGGTGCGGGGGTGGC |
| Internal bar code: | GCGTTAGTGGAATGGCGGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.8944 |
| LEAP-Seq n confirming: | 946 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAGCTACACCGCAACTA |
| Suggested primer 2: | AAAGAGGGAGGACTGTGGGT |