| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.113384 |
| Chromosome: | chromosome 1 |
| Location: | 5276246 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036850 | PDY1 | (1 of 2) 1.5.5.2 - Proline dehydrogenase; Proline dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGAGCTCGTGGCAGCAGCGCTGGAGGG |
| Internal bar code: | GCATGGCTCGGCTTGCTCAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTACGAAGGTCCCGTTAG |
| Suggested primer 2: | TAACGAGGCGCAGTACACAC |