Insertion junction: LMJ.RY0402.113668_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.113668
Chromosome:chromosome 7
Location:1235129
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATAATTTCAAGTGTGTGAACCGTGTGTCTG
Internal bar code:GCAACCGCAGCGCTGGTGAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:521
LEAP-Seq percent confirming:99.3789
LEAP-Seq n confirming:2720
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGGTAGCGTGATTTCGTT
Suggested primer 2:TGCCTGTCTTCAGAGGTGTG