Insertion junction: LMJ.RY0402.113668_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.113668
Chromosome:chromosome 7
Location:1235134
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGAAGACAGCGTCAGGCATGCGGTACCC
Internal bar code:GGGCAAAACTGCGCCGTGCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:887
LEAP-Seq percent confirming:99.7486
LEAP-Seq n confirming:1587
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGGTAGCGTGATTTCGTT
Suggested primer 2:TGCCTGTCTTCAGAGGTGTG