Insertion junction: LMJ.RY0402.114155_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.114155
Chromosome:chromosome_3
Location:3048867
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre03.g164250 FAP262 Flagellar Associated Protein, protein kinase sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TGCGAGGAGGGAGGAGAAAGGGGGGGGGGC
Internal bar code:GCACGCATGATGCCGGATTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:890
LEAP-Seq percent confirming:99.7074
LEAP-Seq n confirming:1704
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAAATGGTGTACCGCGAC
Suggested primer 2:CTCCTCCGCTTCTGCTACCT