Insertion junction: LMJ.RY0402.114155_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.114155
Chromosome:chromosome_3
Location:3048871
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre03.g164250 FAP262 Flagellar Associated Protein, protein kinase sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):ATGTGGGCCATCGGCTGCCTCATGGGCGAG
Internal bar code:CGCCCAGGGACCTACACGCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:370
LEAP-Seq percent confirming:99.7382
LEAP-Seq n confirming:762
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAAATGGTGTACCGCGAC
Suggested primer 2:CTCCTCCGCTTCTGCTACCT