| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114272 |
| Chromosome: | chromosome 13 |
| Location: | 1673029 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574000 | GEF1,CLV1 | Voltage-gated chloride channel; (1 of 1) PTHR11689:SF59 - CHLORIDE TRANSPORT PROTEIN 6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGGGAACTACACCCGAGGCTGGAGTGC |
| Internal bar code: | GTAGCAGTTTAGCTCCTAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 99.6423 |
| LEAP-Seq n confirming: | 3064 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTCAGGTCGAGGATGA |
| Suggested primer 2: | TGTCTTGGAGCACGAGTACG |