Insertion junction: LMJ.RY0402.115091_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.115091
Chromosome:chromosome_3
Location:7304185
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g206900 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):AGACCCAACCGCGGCCCTCCGCCGCCTCCG
Internal bar code:GCAAAGGGCAACAAACGGCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:794
LEAP-Seq percent confirming:87.5648
LEAP-Seq n confirming:169
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCCACTTGTTGTGAACTC
Suggested primer 2:TTGATGCGTTGACGAGTAGG